We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A synthetic luciferin improves bioluminescence imaging in live mice.
- Authors
Evans, Melanie S; Chaurette, Joanna P; Adams, Spencer T; Reddy, Gadarla R; Paley, Miranda A; Aronin, Neil; Prescher, Jennifer A; Miller, Stephen C
- Abstract
Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice with a synthetic luciferin, CycLuc1, improves BLI with existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin.
- Subjects
BRAIN imaging; LUCIFERASES; MOLECULAR biology; LABORATORY mice; PHARMACOLOGY; BIOLUMINESCENCE
- Publication
Nature Methods, 2014, Vol 11, Issue 4, p393
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.2839