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- Title
Effects of some parameters in upscale culture of Haematococcus pluvialis Flotow.
- Authors
Sipaúba-Tavares, L. H.; Millan, R. N.; Berchielli-Morais, F. A.
- Abstract
Growth rate and medium parameters between two bench scale volumes ( 13-L and 250-L) were compared. Experiments were maintained batch mode and culture parameters were periodically measured during a 13-day period. Culture growth during the cultivation of algae Haematococcus pluvialis was determined qualitatively by cell counting, optical density, dry weight, ash content, amount of chlorophyll-a, total organic carbon content and by direct measuring of me-dium nutrients and some abiotic aspects. Vegetative cell growth was higher when cultured in 13-L with 1.33 x 105 cells.mL-1 on the 12th day than when cultured in 250-L. Significant difference (p < 0.05) in the biology and water cul-ture of H. pluvialis, with the exception of dry weight, ash, nitrite and ammonia, was reported between the volumes. Data obtained in current study for the upscale culture maintenance of H. pluvialis in laboratory conditions shows that it should be undertaken in a 13-L volume due to a greater time span of cells in a vegetative state, greater cell density, lipids and chlorophyll-a contents. Light was of paramount importance on the direct performance of H. pluvialis on the algal biological conditions.
- Subjects
GREEN algae; ALGAE culture; ALGAL growth; FLOW cytometry; OPACITY (Optics); CHLOROPHYLL; MICROALGAE
- Publication
Brazilian Journal of Biology, 2013, Vol 73, Issue 3, p585
- ISSN
1519-6984
- Publication type
Article