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- Title
POTENTIAL USE OF LECTINS TO IDENTIFY INTACT AND RUPTURED TRICHOSTRONGYLE NEMATODE EGGS IN SHEEP FECAL SAMPLES.
- Authors
Al-Kappany, Y. M.; Sarah, A.; Hildreth, M. B.
- Abstract
Diagnosing trichostrongyle infections in sheep and goats is difficult because the diagnostic egg stage of most trichstrongyle genera is morphologically indistinguishable. The lectin, peanut agglutinin (PNA), has been shown to bind to the external surface of Haemonchus eggs but not to the eggs of 2 other trichostrongyle genera commonly found in sheep and goats (i.e. Trichstrongylus and Theladorsagia). The binding of 20 other lectins to the outer surface of Haemonchus and Trichstrongylus and/or Theladorsagia eggs has already been evaluated, but none have shown the type of specificity observed with PNA. The only egg component used in lectin studies thus far has been the outer egg surface. By freezing eggs at -40 °C for 2 hours prior to lectin staining, it is possible to rupture the egg shell, and thus simultaneous evaluate the binding of lectins to the outer and inner egg shell surfaces, and to the embryonic blastomeres. The present study evaluated the binding of 20 lectins in the 3 screening kits sold by ©Vector Laboratories, Inc. (i.e. ECL, GSL-I&II, AIL, PNA, RCA, SBA, DBA, VVA, ConA, LCA, PSA, WGA, SWGA, DSL, LEL, STL, UEA, PHA-L&E) to the ruptured fecal eggs isolated from a ewe naturally infected with Haemonchus contortus, Theladorsagia circumcinta, Ostertagia leptospicularis, Trichstrongylus colubriformis, T. vitrinus, T. rugatus and T. axei. Only ConA and STL (Solanum tuberosum lectin) bound to blastomeres from any of the eggs. PNA and STL bound intensely, and GSL-I, AIL, ConA, and SWGA bound moderately to shells from at least some of the eggs. These 6 lectins should be further evaluated to determine if their binding is specific for each genus or species of trichostrongyle.
- Subjects
LECTINS; SHEEP infections; HAEMONCHUS
- Publication
Proceedings of the South Dakota Academy of Science, 2015, Vol 94, p376
- ISSN
0096-378X
- Publication type
Article