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- Title
Monosaccharide compositional analysis of marine polysaccharides by hydrophilic interaction liquid chromatography-tandem mass spectrometry.
- Authors
Qiuju Gao; Nilsson, Ulrika; Ilag, Leopold L.; Leck, Caroline
- Abstract
simple and sensitive method was developed using hydrophilic interaction liquid chromatography coupled to tandem mass spectrometry for determination of monosaccharides liberated from marine polysaccharides by acidic hydrolysis. Optimal separation of diastereomeric monosaccharides including hexoses, pentoses, and deoxyhexoses was achieved using an aminopropyl bonded column with mobile phase containing ternary solvents (acetonitrile/methanol/water) in conjunction with MS/MS in SRM mode. Mechanisms for fragmentation of deprotonated monosaccharides with regard to cross-ring cleavage were proposed. Matrix effects from coeluting interferences were observed and isotopic-labeled internal standard was used to compensate for the signal suppression. The method demonstrated excellent instrumental limits of detection (LOD), ranging from 0.7 to 4.2 pg. Method LODs range from 0.9 to 5.1 nM. The proposed method was applied to the analysis of polysaccharides in seawater collected from the open leads of the central Arctic Ocean in the summer of 2008.
- Subjects
MONOSACCHARIDES; POLYSACCHARIDES; LIQUID chromatography; TANDEM mass spectrometry; HYDROLYSIS
- Publication
Analytical & Bioanalytical Chemistry, 2011, Vol 399, Issue 7, p2517
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-010-4638-z