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- Title
In Vitro Susceptibility of Pythium insidiosum to Melaleuca alternifolia, Mentha piperita and Origanum vulgare Essential Oils Combinations.
- Authors
Souza Silveira Valente, Júlia; Oliveira da Silva Fonseca, Anelise; Denardi, Laura; Dal Ben, Vanessa; Souza Maia Filho, Fernando; Baptista, Cristiane; Braga, Caroline; Zambrano, Cristina; Alves, Sydney; Avila Botton, Sônia; Pereira, Daniela
- Abstract
Pythium insidiosum is the etiologic agent of pythiosis, a severe and emerging disease that affects mammals. Failure of conventional antifungal therapies is partially justified by the absence of ergosterol in the plasma membrane of this oomycete. Despite research advancement, the treatment of pythiosis has not been not fully established. The present study investigated the in vitro susceptibility profile of Brazilian isolates of P. insidiosum ( n = 20) against Melaleuca alternifolia, Mentha piperita and Origanum vulgare essential oils, and their combinations. Susceptibility tests were performed according to CLSI M38-A2 protocol, and combinations were evaluated by the microdilution cherkerboard method. All tested essential oils showed antimicrobial activity against P. insidiosum, and the greatest activity of O. vulgare was highlighted. Synergistic and/or indifferent effect was observed for all combinations evaluated, especially the M. piperita and O. vulgare combination, which showed 65 % synergism. This is the first study to report in vitro combinations of essential oils against P. insidiosum indicating the susceptibility of this oomycete to M. alternifolia, M. piperita and O. vulgare essential oils, as well as their combinations.
- Subjects
PYTHIUM; PYTHIUM diseases; MELALEUCA; ORIGANUM; OREGANO
- Publication
Mycopathologia, 2016, Vol 181, Issue 7/8, p617
- ISSN
0301-486X
- Publication type
Article
- DOI
10.1007/s11046-016-0019-2