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- Title
Overexpression of a <i>Catharanthus</i> tryptophan decarboxylase (tdc) gene leads to enhanced terpenoid indole alkaloid (TIA) production in transgenic hairy root lines of <i>Rauwolfia serpentina</i>.
- Authors
Mehrotra, Shakti; Srivastava, Vikas; Rahman, Laiq Ur; Kukreja, A. K.
- Abstract
To enhance the production of terpenoid indole alkaloids in Rauwolfia serpentina, Catharanthus tryptophan decarboxylase ( Crtdc) gene was over-expressed in transgenic hairy root cultures using Agrobacterium rhizogenes-mediated transformation. Among six transgenic hairy root lines, line RT4 accumulated the highest alkaloid content, with 0.1202 % dry weight (DW) reserpine and 0.0064 % DW ajmalicine, after 10 weeks of culture. Whereas, wild-type roots accumulated 0.0596 ± 0.003 % DW reserpine and 0.0011 ± 0.001 % DW ajmalicine. Transgenic hairy root line RT7 produced the lowest alkaloid content (reserpine: 0.0896 ± 0.002 % DW; ajmalicine: 0.002 ± 0.0 % DW). On the basis of alkaloid content the six hairy root lines were grouped as RT4/RT2 > RT3/RT5 > RT7/RT8. Analysis of gene expression profile indicated that Crtdc was expressed at a higher level in transgenic lines, which could be correlated with enhanced metabolite accumulation in roots. This study confirms that over-expression of Crtdc is a superlative method to improve the biosynthetic potential of Rauwolfia hairy root cultures. Enhanced reserpine and ajmalicine production can serve as an alternative choice to provide resources for relative pharmaceutical industries.
- Subjects
CATHARANTHUS; AROMATIC amino acid decarboxylases; GENE expression in plants; TERPENES; ALKALOIDS; RAUVOLFIA serpentina
- Publication
Plant Cell, Tissue & Organ Culture, 2013, Vol 115, Issue 3, p377
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-013-0369-0