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- Title
ATP dynamic regeneration strategy for enhancing co-production of glutathione and S-adenosylmethionine in Escherichia coli.
- Authors
Chen, Ya Wei; Liao, Yuan; Kong, Wei Zhen; Wang, Shu Han
- Abstract
Objectives: In general, a sufficient supply of ATP can promote the synthesis of ATP-driven metabolites, but excessive ATP will lead to the inhibition of cell growth. For enhancing the co-production of glutathione(GSH) and S-adenosylmethionine(SAM), a dynamic ATP regeneration strategy was developed. Results: The novel ATP regeneration strategy consisting of ATP-sensing riboswitch ydaO motif, polyphosphate kinase (PPK), and Vitreoscilla hemoglobin (VHb) was successfully applied in Escherichia coli. The intracellular ATP level was always around 0.60 mg/g dry cell weight during the fermentation process, resulting in significantly enhanced co-production of GSH and SAM. The GSH titer and SAM titer in the strain CGS-2 increased by 137.40% and 82.18% after fermentation for 24 h, compared with the control strain. Conclusions: The ATP regulation strategy is expected to be a favorable tool to improve the efficiency of microbial cell factories. The proposed ATP dynamic regeneration approach may be applicable for cost-effective, high-yield production of ATP-driven metabolites.
- Subjects
ADENOSYLMETHIONINE; ESCHERICHIA coli; MICROBIAL cells; ELECTRIC batteries; CELL growth
- Publication
Biotechnology Letters, 2020, Vol 42, Issue 12, p2581
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-020-02989-9