We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Elevated 3-hydroxypropionaldehyde production from glycerol using a Citrobacter freundii mutant.
- Authors
West, Thomas; Peterson, Jessica
- Abstract
A mutant strain of Citrobacter freundii capable of elevated 3-hydroxypropionaldehyde production from glycerol was isolated using chemical mutagenesis and a screening protocol. The protocol involved screening mutagenized bacterial cells on solid minimal medium containing 5 % (v/v) glycerol. Colonies were picked onto duplicate solid minimal medium plates and one plate was stained with 1 % (w/v) phloroglucinol. Those colonies staining red were further screened and a mutant, HPAO-1, was identified. The mutant strain produced a several-fold higher 3-hydroxypropionaldehyde concentration than did the parent strain when grown on 5 % (v/v) glycerol. The ratio of culture volume to flask volume influenced 3-hydroxypropionaldehyde production by the mutant cells compared to the parent cells. Aldehyde production was highest when the mutant strain was grown on 5 % (v/v) glycerol at a ratio of culture volume to flask volume of 1:3 or 1:12.5.
- Subjects
BIOMASS; CITROBACTER; GLYCERIN; PHLOROGLUCINOL; CHEMICAL mutagenesis
- Publication
Biotechnology Letters, 2014, Vol 36, Issue 1, p147
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-013-1343-y