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- Title
Isolation and structural analysis of bamylocin A, novel lipopeptide from Bacillus amyloliquefaciens LP03 having antagonistic and crude oil-emulsifying activity.
- Authors
Sang-Cheol Lee; Sun-Hee Kim; In-Hye Park; Soo-Yeol Chung; Yong-Lark Choi
- Abstract
Bacillus amyloliquefaciens strain LP03 isolated from soil, produced an antagonistic compound that strongly inhibited the growth of plant-pathogenic fungi and a lipopeptide biosurfactant. Also, isolated strain LP03 had a marked crude oil-emulsifying activity as it developed a clear zone around the colony after incubation for 24 h at 37°C. LP03 was identified as Bacillus amyloliquefaciens by analysis of partial 16 S rRNA gene and partial gyrA gene sequence. The lipopeptide was purified by acid precipitation of cell-free culture broth, extraction of the precipitates with methanol, silica gel column chromatography, and reverse-phase, high-pressure liquid chromatography. The purified biosurfactant was analyzed biochemical structure by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) and electrospray ionization mass spectrometry/mass spectrometry (ESI-MS/MS). The masses of the two peaks were observed by HPLC chromatography. Their masses were determined to be 1,044 and 1,058 m/ z with MALDI-TOF mass spectrometry. As constituents of the peptide and lipophilic part of the m/ z 1,022.6, seven amino acids (Glu-Leu-Met-Leu-Pro-Leu-Leu) and β-hydroxy-C13 fatty acid were determined by ESI-MS/MS. The lipopeptide of 1,022.6 Da differed from surfactins in the substitution of leucine, valine and aspartic acid in positions 3, 4, and 5 by methionine, leucine, and proline, respectively. Novel lipopeptide was designated as bamylocin A.
- Subjects
PEPTIDES; STRUCTURAL analysis (Science); GROWTH factors; BACILLUS (Bacteria); ANTAGONISTIC fungi; PETROLEUM; BIOSURFACTANTS; MICROBIAL surfactants; PLANT growth
- Publication
Archives of Microbiology, 2007, Vol 188, Issue 4, p307
- ISSN
0302-8933
- Publication type
Article
- DOI
10.1007/s00203-007-0250-9