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- Title
Boric Acid Is Reproductively Toxic to Adult Xenopus laevis, but Not Endocrine Active.
- Authors
Fort, Douglas J.; Fort, Troy D.; Mathis, Michael B.; Ball, R. Wayne
- Abstract
The potential reproductive and endocrine toxicity of boric acid (BA) in the African clawed frog, Xenopus laevis, was evaluated using a 30-day exposure of adult frogs. Adult female and male frogs established as breeders were exposed to a culture water control and 4 target (nominal) test concentrations [5.0, 7.5, 10.0, and 15mg boron (B)/L, equivalent to 28.5, 42.8, 57.0, and 85.5mg BA/L] using flow-through diluter exposure system. The primary endpoints measured were adult survival, growth (weight and snout-vent length [SVL]), necropsy data, reproductive fecundity, and development of progeny (F1) from the exposed frogs. Necropsy endpoints included gonad weight, gonado-somatic index (GSI), ovary profile (oocyte normalcy and stage distribution), sperm count, and dysmorphology. Endocrine endpoints included plasma estradiol (E2), testosterone (T), dihydrotestosteone (DHT), gonadal CYP 19 (aromatase), and gonadal 5a-reductase (5-AR). BA exposure to adult female X. laevis increased the proportion of immature oocytes (<stage II) in the ovaries of females, reduced sperm counts and increased sperm cell dysmorphology frequency in male frogs exposed to 15mg B/L. No effects on the other general, developmental (F1), or endocrine endpoints were observed. Based on the results of the present study, the no observed adverse effects concentration (NOAEC) for the reproductive endpoints was 10mg B/L; and 15mg B/L for reproductive fecundity, F1 embryo larval development, and endocrine function. These results confirmed that although BA is capable of inducing reproductive toxicity at high concentrations, it is not an endocrine disrupting agent.
- Subjects
BORIC acid; XENOPUS laevis; REPRODUCTIVE toxicology; ENDOCRINE toxicology; AUTOPSY
- Publication
Toxicological Sciences, 2016, Vol 154, Issue 1, p16
- ISSN
1096-6080
- Publication type
Article
- DOI
10.1093/toxsci/kfw138