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- Title
Fertilization and early embryology: Blocking of human fertilization by carbohydrates.
- Authors
Mori, Kazumasa; Daitoh, Toshifumi; Kamada, Masaharu; Maeda, Nobuhiko; Maegawa, Masahiko; Hirano, Kohki; Irahara, Minoru; Aono, Toshihiro
- Abstract
The zona penetration test and triple stain technique were used to elucidate the blocking effects of carbohydrates on human fertilization and their mechanisms. In the presence of D-mannose or D-fructose (final concentration, 50 mmol/1), sperm penetration through the human zona pellucida was completely blocked. The triple stain technique revealed that D-fructose (50 mmol/1) significantly ( < 0.01) suppressed the acrosome reaction of human spermatozoa, while D-mannose did not show a suppressive effect on the acrosome reaction. These results reinforce our hypothesis proposed previously, that a mandatory step in human fertilization is the binding of a D-mannose-binding constituent of the sperm surface to a D-mannose residue in the sperm receptor site on the zona pellucida. In addition, D-fructose may play an important role as an acrosome stabilizing factor in seminal fluid.
- Publication
Human Reproduction, 1993, Vol 8, Issue 10, p1729
- ISSN
0268-1161
- Publication type
Article