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- Title
Fetal cells in the maternal circulation: isolation by multiparameter flow cytometry and confirmation by polymerase chain reaction.
- Authors
Wachtel, S; Elias, S; Price, J; Wachtel, G; Phillips, O; Shulman, L; Meyers, C; Simpson, J L; Dockter, M
- Abstract
During pregnancy, nucleated fetal erythrocytes enter the maternal circulation and can be isolated efficiently from the maternal cells by multiparameter flow cytometry. Male DNA, implying presence of a male fetus, can be identified in flow-sorted maternal blood by polymerase chain reaction with oligonucleotide primers flanking single-copy Y-specific DNA sequences. Among flow-sorted samples, we correctly identified fetal sex in 17/18 (94%) pregnancies of 10-21 weeks gestation. Maternal blood thus provides a potential opportunity for prenatal diagnosis that could preclude the need for invasive procedures in current use.
- Subjects
ERYTHROCYTES; CELL receptors; CELL separation; DOCUMENTATION; FLOW cytometry; GESTATIONAL age; NUCLEOTIDES; POLYMERASE chain reaction; RESEARCH funding; DIAGNOSTIC sex determination; EMBRYOS
- Publication
Human Reproduction, 1991, Vol 6, Issue 9, p1466
- ISSN
0268-1161
- Publication type
journal article