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- Title
Evaluation of Shockwave Lithotripsy Induced Renal Damage Using Heat Shock Protein 70 Expression in the Presence of Different Urinary Obstruction Periods.
- Authors
Bahattin Ulu; Emre Tüzel; Fatma Aktepe; Bülent Akdoğan; Cem Güler
- Abstract
AbstractPurpose:The aim of this study was to investigate the deleterious effects of shockwaves (SWs) using heat shock protein (HSP) 70 expression on obstructed kidneys with different obstruction periods.Materials and Methods:Twenty-five rabbits were divided into two groups with 11 rabbits in each group. Both kidneys of the remaining three rabbits served as a sham group. All animals underwent right ureteral ligation using surgical clips. In group 1 (n = 6), 1500 SWs were applied to the obstructed kidney after 3 days of ligation, and the kidneys were removed on day 4. Group 1 control (n = 5) animals also underwent nephrectomy on day 4 without receiving SWs. Group 2 (n = 6) animals received the same dose of SWs after 6 days of ureteral ligation and were nephrectomized on day 7. Group 2 control (n = 5) animals did not receive SWs and were nephrectomized on day 7. Immunohistochemical staining was performed for HSP70 expression, and staining intensity was graded semiquantatively.Results:HSP70 staining results were significantly lower in the sham group compared with results in the study groups. Immunostaining was significantly higher in the cortical collector tubuli in group 1 than group 1 control (P= 0.006). No significant difference was detected in group 2 compared with group 2 control. In the medullar cortical tubuli, HSP70 staining was significantly higher in group 1 than group 1 control (P= 0.03). It was also higher in group 2 than group 2 control (P= 0.04). The mean HSP70 glomerular staining scores were not statistically significant between each group.Conclusion:Application of SWs in the presence of obstruction has deleterious effects on the kidney tissue.
- Subjects
EXTRACORPOREAL shock wave lithotripsy; HEAT shock proteins; GENE expression; URINARY obstructions; LABORATORY rabbits; TISSUE analysis; SURGICAL complications
- Publication
Journal of Endourology, 2009, Vol 23, Issue 7, p1093
- ISSN
0892-7790
- Publication type
Article
- DOI
10.1089/end.2008.0638