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- Title
Generation and characterization of functional cardiomyocytes using induced pluripotent stem cells derived from human fibroblasts
- Authors
Gai, Hui; Leung, Elaine Lai-Han; Costantino, Peter D.; Aguila, Jerell R.; Nguyen, David M.; Fink, Louis M.; Ward, David C.; Ma, Yupo
- Abstract
Abstract: We have successfully developed both spontaneous and inductive cardiomyocyte differentiation of iPS cells reprogrammed from human foreskin fibroblasts. The reprogrammed iPS cells morphologically resemble human cardiomyocytes which can beat. RT-PCR and immunostaining show that cardiac markers are expressed that are comparable to the differentiation pattern of authentic human embryonic stem cells, indicating the existence of both immature and mature differentiated cardiomyocytes. 5-Azacytidine greatly enhanced the efficiency of cardiomyocyte differentiation, whereas dimethylsulfoxide had no effect. Low serum and bone morphogenetic protein-2 marginally improved differentiation efficiency. iPS cell-derived cardiomyocytes changed their beat frequency in response to cardiac drugs, which included ion channel blockers and α/β adrenergic stimulators. Derived cardiomyocytes look promising as an in vitro system for potential drug screen and/or toxicity, making this system closer to practical use in the near future.
- Subjects
HEART cells; CELL differentiation; FIBROBLASTS; EMBRYONIC stem cells; SOMATIC cells; DRUG toxicity; REVERSE transcriptase polymerase chain reaction; BONE morphogenetic proteins; CARDIOVASCULAR agents
- Publication
Cell Biology International, 2009, Vol 33, Issue 11, p1184
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1016/j.cellbi.2009.08.008