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- Title
The glomerular parietal epithelial cell's responses are influenced by SM22 alpha levels.
- Authors
Shokichi Naito; Pippin, Jeffrey W.; Shankland, Stuart J.
- Abstract
Background Studies have shown in several diseases initially affecting podocytes, that the neighboring glomerular parietal epithelial cells (PECs) are secondarily involved. The PEC response might be reparative under certain circumstances, yet injurious under others. The factors governing these are not well understood. We have shown that SM22α, an actin-binding protein considered a marker of smooth muscle differentiation, is upregulated in podocytes and PECs in several models of podocyte disease. However, the impact of SM22α levels on PECs is not known. Methods Experimental glomerular disease, characterized by primary podocyte injury, was induced in aged-matched SM22α +/+ and SM22α -/- mice by intraperitoneal injection of sheep antirabbit glomeruli antibody. Immunostaining methods were employed on days 7 and 14 of disease. Results The number of PEC transition cells, defined as cells co-expressing a PEC protein (PAX2) and podocyte protein (Synaptopodin) was higher in diseased SM22α-/- mice compared with SM22α+/+ mice. WT1 staining along Bowman's capsule is higher in diseased SM22α -/- mice. This was accompanied by increased PEC proliferation (measured by ki-67 staining), and an increase in immunostaining for the progenitor marker NCAM, in a subpopulation of PECs in diseased SM22α -/- mice. In addition, immunostaining for vimentin and alpha smooth muscle actin, markers of epithelial-to-mesenchymal transition (EMT), was lower in diseased SM22α -/- mice compared to diseased SM22α+/+ mice. Conclusion SM22α levels may impact how PECs respond following a primary podocyte injury in experimental glomerular disease. Absent/lower levels favor an increase in PEC transition cells and PECs expressing a progenitor marker, and a lower EMT rate compared to SM22α+/+ mice, where SM22 levels are markedly increased in PECs.
- Subjects
EPITHELIAL cells; REGENERATION (Biology); KIDNEY glomerulus; PROGENITOR cells; GLOMERULONEPHRITIS
- Publication
BMC Nephrology, 2014, Vol 15, Issue 1, p1
- ISSN
1471-2369
- Publication type
Article
- DOI
10.1186/1471-2369-15-174