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- Title
高效液相色谱-串联质谱法 测定婴幼儿奶粉中核苷酸.
- Authors
陈晶燕; 陈万勤; 王峰; 陈碧莲; 周霞; 刘柱; 梁晶晶
- Abstract
Nucleotides are biologically important small molecules composed of a nitrogenous base. a five-carbon sugar (ribose or deoxyribose) and at least one phosphate group. They play important role in improving gastrointestinal tract repair after damage. maintaining the immune system and regulating lipoprotein metabolism. Nowadays the role of dietary nucleotides has been paid increased attention in infant nutrition. Dietary nucleotides are crucial to maintaining normal growth and development in infants. There fore the contents of nucleotides in infant milk powder become important. A method for the determination of five nucleotides (cyridine 5 monophosphate (CMP), guanosine 5 monophosphate (GMP), uridine monophosphate (UMP), adenosine t-monophos- phate (AMP) and inosine 5'- monophosphate (IMP) in infant milk powder by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed. The nucleotides in sample were hydrolysed by alkaline phosphatase to the corresponding nucleosides. The nucleosides were adsorbed by selective boric acid solid phase extraction. After purification and elution. the chromatographic separation was performed on an ACQUITY UPLC HSS T3 column (2.1 mm x 100 mmx1.8 μm), and gradient eluted by 5 mmol/L ammonium formate and acetonitrile, and analyzed by HPLC-MS/MS in multiple reaction monitoring (MRM) mode via positive electrospray ionization, The contents of nucleotides were quantitatively analyzed by internal standard method. The results showed that the linear ranges of five nucleotides in infant formula are good, the correlation coefficients are above 0, 998. Five kinds of nucleosides can be separated within 8 min. Cytidine, inosine and guanosine are all linear in the concentra tion range of 5-200 μg/L. adenosine and uridine are linear in the concentration range of 1-40 μg/L and 25-1 000 μg/L. respectively, The recoveries are between 74.2% and 110.7% with the relative standard deviations from 0. 8% to 5. 3% (n=6). The limits of quantitation of CMP, CMP, GMP. UMP and IMP are 0. 7, 0.1, 0. 7, 3. 3, 0. 6 mg/100 g. respectively. In order to study the accuracy of the method. 3 infant milk powder were analyzed. The method is sensitive. accurate. has good reproducibility and is suitable for the simultaneous determination of nucleotide content in infant formula products.
- Subjects
LIQUID chromatography-mass spectrometry; BASE pairs; SOLID phase extraction; SMALL molecules; INFANT nutrition; COMPOSITION of breast milk; NUCLEOTIDES; URIDINE
- Publication
Journal of Chinese Mass Spectrometry Society, 2020, Vol 41, Issue 6, p93
- ISSN
1004-2997
- Publication type
Article
- DOI
10.7538/zpxb.2019.0156