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- Title
Role of immunostaining in detecting extra-pattern and subtle lymphomatous infiltration in bone marrow biopsies of non- Hodgkin lymphoma patients.
- Authors
Hassan, Noha Bassiouny; El Sakhawy, Yasmin N.; Hamed, Gehan M.
- Abstract
Introduction: Immunohistochemistry (IHC) enables the examination of a greater number of trephine biopsy levels and is helpful in determining additional scattered malignant cells. The aim of this study was to detect extra- pattern and subtle lymphomatous infiltration in bone marrow biopsies of Non Hodgkin Lymphoma (NHL) patients using CD20 and CD3 immunostaining. Patients and Methods: This study was conducted on 100 newly diagnosed NHL patients. Their bone marrow trephine biopsies were assessed on routine histology [Hematoxylin and Eosin (H & E)], and were further subjected to IHC using CD20 and CD3. Results: Pattern of involvement by H & E was highlighted by IHC. It showed additional interstitial pattern in 9 cases, parasinusoidal streaks in one case and highlighted a patchy pattern in another case with interstitial involvement on H & E. IHC also detected subtle infiltrations on additional 5.5% cases compared with histology alone. It helped in differentiating reactive (12 cases) and malignant lymphoid infiltration (33 cases). Conclusion: CD20 and CD3 immunostaining performed routinely on bone marrow trephine biopsies has the ability to reveal extra-pattern of infiltration and improve detection of subtle lymphoid involvement. A combined procedure identifying several distinctive features, in particular histotopography and IHC, provides a promising way of discriminating reactive from neoplastic lymphoid infiltrates in bone marrow trephine biopsies.
- Publication
Journal of Applied Hematology, 2019, Vol 10, pS40
- ISSN
1658-5127
- Publication type
Article