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- Title
Identification of a calcium-controlled negative regulatory system affecting Vibrio cholerae biofilm formation.
- Authors
Bilecen, Kivanc; Yildiz, Fitnat H.
- Abstract
Vibrio cholerae's capacity to cause outbreaks of cholera is linked to its survival and adaptability to changes in aquatic environments. One of the environmental conditions that can vary in V. cholerae's natural aquatic habitats is calcium (Ca+2). In this study, we investigated the response of V. cholerae to changes in extracellular Ca2+ levels. Whole-genome expression profiling revealed that Ca2+ decreased the expression of genes required for biofilm matrix production. Luria–Bertani (LB) medium supplemented with Ca2+ (LBCa2+) caused V. cholerae to form biofilms with decreased thickness and increased roughness, as compared with biofilms formed in LB. Furthermore, addition of Ca2+ led to dissolution in biofilms. Transcription of two genes encoding a two-component regulatory system pair, now termed lcium- egulated ensor ( carS) and egulator ( carR), was decreased in cells grown in LBCa2+. Analysis of null and overexpression alleles of carS and carR revealed that expression of vps ( ibrio oly accharide) genes and biofilm formation are negatively regulated by the CarRS two-component regulatory system. Through epistasis analysis we determined that CarR acts in parallel with HapR, the negative regulator of vps gene expression.
- Subjects
MICROBIOLOGY; VIBRIO cholerae; CHOLERA; GENE expression; AQUATIC habitats; MICROBIAL aggregation
- Publication
Environmental Microbiology, 2009, Vol 11, Issue 8, p2015
- ISSN
1462-2912
- Publication type
Article
- DOI
10.1111/j.1462-2920.2009.01923.x