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- Title
COMPARATIVE STUDY ON PRODUCTION OF LIPASE BY BACTERIA AND FUNGI FROM WASTE COOKING OIL.
- Authors
Daware, Rasika R.; Wasnik, Durgesh D.; Bhandari, P. R.; Tumane, P. M.
- Abstract
The lipases are widely distributed in nature and can be derived from various sources such as animals, plants and microorganisms. The major advantages of microbial lipases are commercially significant and the production is important from the economic and industrial standpoints. This present investigation reveals that comparative study on production of microbial lipase from waste cooking oil by Bacillus subtilis, Bacillus thuringiensis and Aspergillus niger. Total four bacterial isolates were isolated on HiChrome Bacillus Agar and fungal isolate was isolated on Potato Dextrose Agar from soil sample by serial dilution method and the rapid identification on the basis of morphological, cultural and biochemical characteristics. These four bacterial strain confirmed Bacillus subtilis, Bacillus thuringiensis, Bacillus megaterium, Bacillus cereus and fungal strain confirmed Aspergillus niger. The primary screening done by plating on tributyrin agar plates led to the finding of two bacterial species i.e. Bacillus subtilis and Bacillus thuringiensis and fungal species i.e. Aspergillus niger classified as maximum lispase producers. Submerged fermentation using waste cooking oil was carried out at 37°C and optimum pH 7.0 for different time periods (24, 48, 72, 96, 144 and 168 hr). Comparison between bacterial and fungal strains, bacterial isolates showed good results with waste cooking oil. Bacterial isolates i.e. Bacillus subtilis showed maximum yield of lipase enzyme was 56 U/ml and Bacillus thuringiensis also gave good results; found to be 53.25 U/ml with waste cooking oil as a substrate. Fungal species i.e. Aspergillus niger also showed a good lipase producer; found to be 33.25U/ml. Among all studied Bacillus and fungal species, the best lipase producer was identified as Bacillus subtilis. The future work deals with its partial purification and application so as to explore cost-effective usage of enzymes.
- Subjects
LIPASES; VEGETABLE oils
- Publication
Journal of Advanced Scientific Research, 2020, Vol 11, p243
- ISSN
0976-9595
- Publication type
Article