We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The Detection of BRAF-V600E Mutation in Hairy Cell Leukaemia by Polymerase Chain Reaction and Study of its associated Clinicohaematological Parameters.
- Authors
Iqbal, Muhammad Ijaz; Robert, Helen Mary; Khan, Nabeela; Mahmood, Rafia; Khurshid, Ayesha; Khan, Saleem Ahmed
- Abstract
Objective: To contemplate the occurrence of a rare mutation (BRAF V600E) in the patients having Hairy cell leukemia in our setting, which wouldserve asuseful analytic and diagnostic criteria. Study Design: Analytical cross sectional study. Place and Duration of Study: Armed Forces Institute of Pathology Rawalpindi Pakistan, from Oct 2017 to Oct 2018. Methodology:A sample of 36 patients with a suspicion of Hairy cell leukemia were included in this investigation. Complete blood counts, bone marrow examination (trephine biopsy and aspiration), cyto-chemistry and staining of the patients suspected to have Hairy cell leukemia was performed. PCR was done for detection of BRAF V600E mutation. Results: 29 (80.5%) cases showed a positive BRAF mutational analysis and 7 (19.4%) cases showed a negative result. Conclusion:Almost all the cases of classic Hairy cell leukemia had BRAF V600 E mutation.Isolating this mutation can be used as a regular tool in detection of disease and monitoring disease progression and relapse.
- Subjects
POLYMERASE chain reaction; BONE marrow examination; BLOOD cell count; LEUKEMIA; DISEASE relapse
- Publication
Pakistan Armed Forces Medical Journal, 2022, Vol 72, pS178
- ISSN
0030-9648
- Publication type
Article
- DOI
10.51253/pafmj.v72isuppl-2.3083