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- Title
Bioactive Sphene-Based Ceramic Coatings on cpTi Substrates for Dental Implants: An In Vitro Study.
- Authors
Elsayed, Hamada; Brunello, Giulia; Gardin, Chiara; Ferroni, Letizia; Badocco, Denis; Pastore, Paolo; Sivolella, Stefano; Zavan, Barbara; Biasetto, Lisa
- Abstract
Titanium implant surface modifications have been widely investigated to favor the process of osseointegration. The present work aimed to evaluate the effect of sphene (CaTiSiO5) biocoating, on titanium substrates, on the in vitro osteogenic differentiation of Human Adipose-Derived Stem Cells (hADSCs). Sphene bioceramic coatings were prepared using preceramic polymers and nano-sized active fillers and deposited by spray coating. Scanning Electron Microscopy (SEM) analysis, surface roughness measurements and X-ray diffraction analysis were performed. The chemical stability of the coatings in Tris-HCl solution was investigated. In vitro studies were performed by means of proliferation test of hADSCs seeded on coated and uncoated samples after 21 days. Methyl Thiazolyl-Tetrazolium (MTT) test and immunofluorescent staining with phalloidin confirmed the in vitro biocompatibility of both substrates. In vitro osteogenic differentiation of the cells was evaluated using Alizarin Red S staining and quantification assay and real-time PCR (Polymerase Chain Reaction). When hADSCs were cultured in the presence of Osteogenic Differentiation Medium, a significantly higher accumulation of calcium deposits onto the sphene-coated surfaces than on uncoated controls was detected. Osteogenic differentiation on both samples was confirmed by PCR. The proposed coating seems to be promising for dental and orthopedic implants, in terms of composition and deposition technology.
- Subjects
SPHENE; CERAMIC coating; DENTAL implants; CALCIUM compounds; TITANIUM; SUBSTRATES (Materials science)
- Publication
Materials (1996-1944), 2018, Vol 11, Issue 11, p2234
- ISSN
1996-1944
- Publication type
Article
- DOI
10.3390/ma11112234