We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
DEVELOPING AND TAILORING PROTOCOLS FOR TRANSDIFFERENTIATION OF MUSCLE SATELLITE CELLS TO ADIPOCYTES IN RODENT EXPERIMENTS.
- Authors
Oboroceanu, Teodor; Hristov, Ioana; Pînzariu, Alin Constantin; Mocanu, Veronica
- Abstract
In our studies, we focused on pathogenic mechanism involved in transdifferentiating myoblasts (satellite cells) in adipocytes. Transdifferentiation of skeletal muscle into adipocytes could lead to sarcopenia or insulin resistance. Adipocyte differentiation of muscle precursors could be used as an in vitro cell culture model for studying the various interventions that could inhibit this transformation. We present our experience and protocol to collect, extract, characterize, proliferate and transdifferentiate the satellite cells in adipocytes in rodents experiments. The knowledge could be translated on human satellite cells studies in older adults with frailty.
- Subjects
SATELLITE cells; MUSCLE cells; FAT cells; MYOBLASTS; RODENTS; SKELETAL muscle
- Publication
Acta Medica Marisiensis, 2019, Vol 65, p30
- ISSN
2068-3324
- Publication type
Article