We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The Structural Organization of Ascidian Halocynthia roretzi Troponin I Genes1.
- Authors
Yuasa, Hajime Julie; Kawamura, Kazuo; Yamamoto, Hiroaki
- Abstract
The organization of troponin I (Tnl) genes from the ascidian Halocynthia roretzi have been determined. Halocynthia possesses roughly two types of TnI isoforms. One type is a single-copied adult TnI (adTnI) gene, which contains eight exons and seven introns. adTnl expresses two isoforms, the shorter body wall muscle TnI and the longer cardiac Tnl, through alternative splicing. The mRNAs of these TnI isoforms may undergo transplicing of the 5‘-leader sequences, like the TnI mRNA of another ascidian species, Ciona intestinalis. The other type comprises multi-copied larval Tnl (laTnI) genes. Halocynthia has at least three laTnIs (α,β and γ), which are composed of five exons and four introns, and two of them (α and γ) are clustered in tandem. All laTnls have B- and M-regions within their 5'-upstream regions, which have been discovered to be the regulatory elements of Halocynthia larval actin genes. The expression of Halocynthia laTnIs and larval actins may be regulated in the same manner. It is known that Ciona does not possess a larva-specific TnI isoform. The phylogenetic tree of ascidian Tnls suggests that laTnls might have only been generated within the Pleurogona lineage after Enterogona/Pleurogona divergence, and this scenario well agress with the absence of laTnIs in Ciona
- Subjects
TROPONIN; MICROFILAMENT proteins; HALOCYNTHIA roretzi; MESSENGER RNA; RNA
- Publication
Journal of Biochemistry, 2002, Vol 132, Issue 1, p135
- ISSN
0021-924X
- Publication type
Article
- DOI
10.1093/oxfordjournals.jbchem.a003191