We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Identification of Five Embryonic Hemoglobins of Rat and Ontogeny of Their Constituent Globins during Fetal Development.
- Authors
Iwahara, Shin-ichiro; Abe, Yasuko; Okazaki, Taro
- Abstract
Hemoglobins of rats switch from an embryonic to an adult type during fetal development. However, very little is known about the structures and molecular species of hemoglobins occurring in the fetal life of rats. In the present study we isolated five embryonic hemoglobins, designated El, E2, E3, E4, and E5, from the blood of rat fetuses on day 14 of gestation by ion exchange chromatography. Reverse-phase high performance liquid chromatography revealed that these hemoglobins each consist of two kinds of globins: E1(11α:ε1), E2(1α:ε1), E3(ζ:ε1), E4(1α:ε3), and E5(ζ:ε3), respectively. The complete ami no acid sequences of the ζ, ε1, and ε3 globins were determined. The ζ globin showed characteristic features common in α-type embryonic globins of known species in that the N-terminus is blocked and the amino acid at position 38 is Gin. ε1 and ε3 are β-type embryonic globins, sharing 73.7% amino acid homology. Interestingly, they are more similar to the corresponding mouse β-type embryonic globins, y and z, respectively, than to each other, implying that these globins have evolved orthologously from common ancestral proteins. It was also shown that the ζ, ε1, and ε3 globins are almost completely replaced by the adult type α and β globins in the blood of rat fetuses by day 18 of gestation.
- Subjects
HEMOGLOBINS; ONTOGENY; CHROMATOGRAPHIC analysis; PREGNANCY; AMINO acids
- Publication
Journal of Biochemistry, 1996, Vol 119, Issue 2, p360
- ISSN
0021-924X
- Publication type
Back Cover
- DOI
10.1093/oxfordjournals.jbchem.a021248