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- Title
PROTECTIVE EFFECT OF AQUEOUS EXTRACT OF DATE FRUIT AGAINST IN VITRO H<sub>2</sub>O<sub>2</sub>- INDUCED CELL DAMAGES.
- Authors
Asadi-Shekaari, Majid; Rajabalian, Saeed; Gholamhoseinian, Ahmad; Ganjooei, Narges Ashraf; Hoseini, Rafat; Mahmoodi, Majid
- Abstract
Fruits of the date palm (Phoenix dactylifera L. Arecaceae) are consumed in many regions of the world. This fruit has been traditionally used as oriental folk medicine for mothers after childbirth and invalids. In the present study, the protective effect of aqueous extract of date fruit (AE-DF) against hydrogen peroxide H2O2-induced cytotoxicity in HEPG- 2, A172, U937 and PC12 cell lines was investigated. Cytotoxic effects were evaluated by XTT assay. The total antioxidant capacity of AE-DF was measured using the Randox kit. In addition, the protective effects of AE-DF against H2O2- induced apoptosis was investigated using DAPI staining. The total antioxidant capacity of AE-DF was 1.97±0.04 mmol Trolox equivalent/l. The results demonstrated that AE-DF inhibits H2O2-induced cell damage in a concentration dependent manner. The 0.1% AE-DF significantly inhibited the 1.47mM H2O2-induced damage, especially in the A172 cells (p<0.05 to 0.001). Moreover, 10% AE-DF completely inhibited 29.4mM H2O2-induced damage. At the same time, the growth of U937 cells significantly increased in the presence of 10% AE-DF compared to the control cells (p<0.01). Cells treated with 2.94 mM H2O2 exhibited several apoptotic features, while those simultaneously exposed with H2O2 and AE-DF showed the complete inhibition of apoptotic features. These results surest that date fruit may exert protective and proliferative effect against H2O2-induced cytotoxicity.
- Subjects
ANTIOXIDANTS; TRADITIONAL medicine; PLANT extracts; DATE palm; APOPTOSIS; DATES (Fruit); ANTINEOPLASTIC antibiotics; REACTIVE oxygen species
- Publication
Current Topics in Nutraceutical Research, 2008, Vol 6, Issue 2, p99
- ISSN
1540-7535
- Publication type
Article