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- Title
A Rapid Test for Detection of Tribenuron-Methyl Resistance in Sunflower.
- Authors
Dimitrijević, Aleksandra; Imerovski, Ivana; Miladinović, Draganaa; Jocić, Sinišaa; Cvejić, Sandraa; Malidža, Goran; Kovačević, Branislav; Šurlan-Momirović, Gordana
- Abstract
A rapid laboratory test for detection of tribenuron-methyl resistant sunflower genotypes was developed. Four homozygous and four heterozygous tribenuron-methyl resistant hybrids, as well as a susceptible one were grown on MS medium supplemented with different concentrations of herbicide (2.0 jiM, 2.5 jiM, 3.0 jiM, 3.5 jiM and 4.0 M) and with pH either 7 or 8. The effect of medium pH and herbicide concentration on above-ground part and root mass of sunflower seedlings was observed. The test enabled visual discrimination between resistant and susceptible genotypes in 5 days, and discrimination between homozygous and heterozygous resistant genotypes in 12 days. All tested herbicide concentrations and morphological parameters were found to be suitable for discrimination of tribenuron-methyl susceptible genotypes. The best pH and herbicide concentration combination and morphological parameter for discrimination between homozygous and heterozygous resistant sunflower genotypes were 3.0 jiM at pH 7 and root mass, respectively.
- Subjects
TRIBENURON-methyl; GENOTYPES; HERBICIDES; SUNFLOWER seeds; VISUAL discrimination
- Publication
Field & Vegetable Crops Research / Ratarstvo i povrtarstvo, 2016, Vol 53, Issue 1, p1
- ISSN
1821-3944
- Publication type
Article
- DOI
10.5937/ratpov53-8412