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- Title
Altered DNA Methylation and Gene Expression Profiles in Radiation-Induced Heart Fibrosis of Sprague-Dawley Rats.
- Authors
Yao, Ye; Chen, Li-Feng; Li, Jin; Chen, Jing; Tian, Xiao-Li; Wang, Hui; Mei, Zi-Jie; Xie, Cong-Hua; Zhong, Ya-Hua
- Abstract
Radiation-induced heart disease (RIHD) is a serious side effect of radiotherapy for thoracic tumors. Advanced myocardial fibrosis in the late phase of RIHD can lead to myocardial remodeling, heart function impairing and heart failure, resulting in serious clinical consequences, and its pathogenesis remains vague. DNA methylation is one of the important epigenetic mechanisms which often occurs in response to environmental stimuli and is crucial in regulating gene expression. We hypothesized DNA methylation may contribute to pathogenesis in radiation-induced heart fibrosis (RIHF) and altered DNA methylation patterns probably influenced the genes expression in RIHF. In present study, we found genome-wide differences in DNA methylation status and RNA expression were demonstrated and we screened out 44 genes whose altered expression maybe were regulated by CpG island methylation within the gene promoter in RIHF of Sprague-Dawley rat by employing gene expression arrays and human CpG island microarrays. Gene expression and CpG island methylation levels of several candidate genes were further validated. Our investigation provided a new dimension to reveal the specific mechanisms of RIHF and explore the potential therapeutic targets for it.
- Subjects
GENE expression profiling; METHYLATION; DNA methylation; HEART fibrosis; SPRAGUE Dawley rats; DNA methyltransferases; RADIOTHERAPY complications
- Publication
Radiation Research, 2022, Vol 198, Issue 2, p154
- ISSN
0033-7587
- Publication type
Article
- DOI
10.1667/RADE-20-00130.1