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- Title
Early lymphocyte activation molecule defined by the monoclonal antibody MLR-3: biochemical and functional studies.
- Authors
Della, D.; Traversari, C.; Ballinari, D.; Cattoretti, G.; Fontanella, E.; Polli, N.
- Abstract
The MLR-3 monoclonal antibody reacts with activated but not with resting lymphocytes. We report that MLR-3 identifies an early activation molecule since its binding is detectable on T cells 1. 5-2 hr after in vitro activation. Its expression, therefore, does not require DNA synthesis and precedes, by many hours, that of the receptors for interleukin-2 (IL-2R) and transferrin (TF-R). The MLR3 antigen is also found on activated thymocytes (including the large early thymic CD3 subset) and B cells. The majority of T- and B-lymphoblastoid cell lines, as well as the myeloid and erythroid cell lines HL60, GMI and K562, are MLR-3+; conversely, non-haemopoietic cell lines are MLR-3 negative. Seventy percent of B-cell chronic lymphocytic leukaemia and 15% of B non-Hodgkin's lymphomas (BaNHL) are MLR-3+. On tissue sections MLR-3 is reactive with epithelia, sweat glands, hair follicles and Henle's loop but not with vessels, connective, endothelium and many other tissues. In vitro studies show that MLR-3 (1-100 μg/ml) significantly alters the thymidine uptake of mitogen-treated lymphocytes:augumentation is found when T and B cells are induced with TPA- lonomycin and reduction when induced with phytohaemoagglutinin (PHA) or Staphylococcus aureus Cowan strain I (SAC), respectively. On SDS-PAGE, MLR-3 immunoprecipitates a disulphide-linked heterodimer of MW 29,000-35,000: both subunits are glycosylated, phosphorye lated and exhibit a pI of 4.1 and 5.0, respectively. Our data, particularly the in vitro results, suggest that the MRL-3 molecule could have an important role in the early hours of activation for the progression of resting lymphocytes into mitosis.
- Subjects
MONOCLONAL antibodies; LYMPHOCYTES; DNA synthesis; INTERLEUKIN-2; T cells; MITOSIS
- Publication
Immunology, 1988, Vol 64, Issue 4, p593
- ISSN
0019-2805
- Publication type
Article