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- Title
Isolation and Purification of Pentagalloylglucose from Radix Paeoniae Alba by High-speed Counter-current Chromatography.
- Authors
Song Xue-ying; Yin Zhi-shuang; Zhao Yi-song; Wang Qiao
- Abstract
High-speed count-current chromatography (HSCCC) was developed for separation and purification of pentagalloylglucose (PGG) from crude extract of Radix Paeoniae Alba by the twice-step separation. The two of biphasic systems were composed of n-hexane-ethyl acetate-methanol-water at a volume ratio of 0.5:5:1:5 and 0.5:5:0.5:5 in HSCCC, respectively. The upper organic phase was as the stationary phase and the lower aqueous phase as mobile phase. The flow rate was at 2.0 mL/min and revolution speed was at 800 rpm. The effluent was monitored with a UV detector at 280 nm. The HSCCC fractions collected were analyzed by HPLC and the target compound of PGG was separated with a purity of 95.7%. In this study, the HSCCC technique was shown to be a powerful method to purify PGG from crude extract of Radix Paeoniae Alba and could represent a new technological tool to be applicable to the preparation of PGG from this source of products.
- Subjects
PENTAGALLOYLGLUCOSE; CHEMICAL purification; PEONIES; CHROMATOGRAPHIC analysis; SEPARATION (Technology); PLANT extracts; HIGH performance liquid chromatography
- Publication
Natural Product Research & Development, 2012, Vol 24, Issue 3, p303
- ISSN
1001-6880
- Publication type
Article