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- Title
PDGF-BB released in tendon repair using a novel delivery system promotes cell proliferation and collagen remodeling.
- Authors
Thomopoulos, Stavros; Zaegel, Melissa; Das, Rosalina; Harwood, Fredrick L.; Silva, Matthew J.; Amiel, David; Sakiyama-Elbert, Shelly; Gelberman, Richard H.
- Abstract
The purpose of this study was to promote fibroblast proliferation and collagen remodeling in flexor tendon repair through sustained delivery of platelet derived growth factor (PDGF-BB). The release kinetics of PDGF-BB from a novel fibrin matrix delivery system was initially evaluated in vitro. After the in vivo degradation rate of the fibrin matrix was determined using fluorescently tagged fibrin, PDGF-BB was delivered to the site of flexor tendon repair in vivo in a canine model. The effect of PDGF-BB on intrasynovial tendon healing was studied using histology-based assays (cell density, proliferation, and type I collagen expression) and by measuring total DNA levels and reducible collagen crosslink levels. The fibrin matrix delivery system provided sustained release of PDGF-BB in vitro at a rate modulated by the ratio of heparin to growth factor. In vivo, the fibrin matrix remained at the repair site for more than 10 days. Delivery of PDGF-BB led to a qualitative increase in cell density, cell proliferation, and type I collagen mRNA expression. PDGF-BB also led to statistically significant increases in total DNA (20% increase at 7 days, 18% increase at 14 days) and reducible collagen crosslinks (30% increase at 7 days). Sustained delivery of growth factors may be achieved using a novel fibrin-based delivery system. PDGF-BB delivery increased cell proliferation and matrix remodeling and thus may accelerate flexor tendon healing. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25:1358-1368, 2007
- Subjects
FLEXOR tendons; GROWTH factors; TENDON injury healing; FIBROBLASTS; FIBROBLAST growth factors; COLLAGEN; BLOOD platelets
- Publication
Journal of Orthopaedic Research, 2007, Vol 25, Issue 10, p1358
- ISSN
0736-0266
- Publication type
Article
- DOI
10.1002/jor.20444