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- Title
酵素1分子検出から生まれたインフルエンザデジタル アッセイ.
- Authors
田端和仁
- Abstract
Digital assays, which have evolved from single-molecule detection technology, are attracting attention as a new method of measuring individual cells, proteins, and nucleic acids. The digital assays are characterized by their ability to directly measure individual molecules, enabling us to perform absolute quantification without the need for calibration curves and to determine the heterogeneity of a population. We first developed a digital assay for enzymes and applied the principle to digital ELISA and digital influenza assays. We demonstrate that the digital assays for enzymes are not only a new enzymology tools, but also have a wide range of applications such as elucidating the nature of viral populations. We will discuss how these new analytical techniques are opening up uncharted territory.
- Subjects
SINGLE molecules; ENZYMES; INFLUENZA; ENZYMOLOGY; MOLECULES; NUCLEIC acids
- Publication
Seibutsu Butsuri, 2021, Vol 61, Issue 2, p95
- ISSN
0582-4052
- Publication type
Article
- DOI
10.2142/biophys.61.095