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- Title
Effect of Conditioning on the Production of Inulinase by Kluyveromyces marxianus var. bulgaricus through Fed-Batch Fermentation.
- Authors
Cazetta, Marcia Luciana; Monti, Rubens; Contiero, Jonas
- Abstract
The effects of sucrose concentration, pH, temperature and aeration rate on the production of biomass and inulinase (2,1-beta-D-fructan fructanohydrolase, EC 3.2.1.7) were investigated using fed-batch fermentation by Kluyveromyces marxianus var. bulgaricus. The best sucrose concentration for enzyme activity was 10 g L-1, whereas activity was inhibited when the sucrose concentration was 20 g L-1. Enzyme activity was strongly influenced by pH of the culture. pH 5.0 was the most suitable for both enzyme activity and cell growth, achieving 98.04 U mL-1 and 14.05 g L-1, respectively, after 72 hours of cultivation. The highest production of inulinase and biomass was observed at temperatures of 25°C and 30°C, whereas a decrease in activity was observed at 35°C and 40°C. The best aeration rate was 1.0 vvm. The increase in aeration rate or oxygen injection provided no increase in enzyme activity and biomass.
- Subjects
FERMENTATION; BIOCHEMICAL engineering; INDUSTRIAL microbiology; MICROBIOLOGICAL synthesis; SUCROSE; SUGAR; DISACCHARIDES
- Publication
International Journal of Food Engineering, 2008, Vol 4, Issue 1, p1
- ISSN
1556-3758
- Publication type
Article
- DOI
10.2202/1556-3758.1157