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- Title
Intrarenal dopamine deficiency leads to hypertension and decreased longevity in mice.
- Authors
Ming-Zhi Zhang; Bing Yao; Suwan Wang; Xiaofeng Fan; Guanqing Wu; Haichun Yang; Huiyong Yin; Shilin Yang; Harris, Raymond C.; Zhang, Ming-Zhi; Yao, Bing; Wang, Suwan; Fan, Xiaofeng; Wu, Guanqing; Yang, Haichun; Yin, Huiyong; Yang, Shilin
- Abstract
In addition to its role as an essential neurotransmitter, dopamine serves important physiologic functions in organs such as the kidney. Although the kidney synthesizes dopamine through the actions of aromatic amino acid decarboxylase (AADC) in the proximal tubule, previous studies have not discriminated between the roles of extrarenal and intrarenal dopamine in the overall regulation of renal function. To address this issue, we generated mice with selective deletion of AADC in the kidney proximal tubules (referred to herein as ptAadc-/- mice), which led to selective decreases in kidney and urinary dopamine. The ptAadc-/- mice exhibited increased expression of nephron sodium transporters, decreased natriuresis and diuresis in response to l-dihydroxyphenylalanine, and decreased medullary COX-2 expression and urinary prostaglandin E2 excretion and developed salt-sensitive hypertension. They had increased renin expression and altered renal Ang II receptor (AT) expression, with increased AT1b and decreased AT2 and Mas expression, associated with increased renal injury in response to Ang II. They also exhibited a substantially shorter life span compared with that of wild-type mice. These results demonstrate the importance of the intrarenal dopaminergic system in salt and water homeostasis and blood pressure control. Decreasing intrarenal dopamine subjects the kidney to unbuffered responses to Ang II and results in the development of hypertension and a dramatic decrease in longevity.
- Subjects
DOPAMINE; HYPERTENSION; LABORATORY mice; NEUROTRANSMITTERS; REGULATION of natriuresis; DIURESIS; LONGEVITY; PROTEIN metabolism; ENZYME metabolism; ALDOSTERONE; ANIMAL experimentation; BIOLOGICAL transport; COMPARATIVE studies; DRUG receptors; ENZYMES; KIDNEY tubules; KIDNEYS; RESEARCH methodology; MEDICAL cooperation; MEMBRANE proteins; MICE; OXIDOREDUCTASES; PROTEINS; RENIN; RESEARCH; RESEARCH funding; EVALUATION research; ION transport (Biology); ANATOMY
- Publication
Journal of Clinical Investigation, 2011, Vol 121, Issue 7, p2845
- ISSN
0021-9738
- Publication type
journal article
- DOI
10.1172/JCI57324