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- Title
A Novel Podophage StenR_269 Suggests a New Family in the Class Caudoviricetes.
- Authors
Yakubovskij, Vyacheslav I.; Morozova, Vera V.; Kozlova, Yuliya N.; Tikunov, Artem Y.; Babkin, Igor V.; Bardasheva, Alevtina V.; Zhirakovskaya, Elena V.; Baykov, Ivan K.; Kaverina, Galina B.; Tikunova, Nina V.
- Abstract
Stenotrophomonas rhizophila was first discovered in soil; it is associated with the rhizosphere and capable of both protecting roots and stimulating plant growth. Therefore, it has a great potential to be used in biocontrol. The study of S. rhizophila phages is important for a further evaluation of their effect on the fitness and properties of host bacteria. A novel phage StenR_269 and its bacterial host S. rhizophila were isolated from a soil sample in the remediation area of a coal mine. Electron microscopy revealed a large capsid (~Ø80 nm) connected with a short tail, which corresponds to the podovirus morphotype. The length of the genomic sequence of the StenR_269 was 66,322 bp and it contained 103 putative genes; 40 of them encoded proteins with predicted functions, 3 corresponded to tRNAs, and the remaining 60 were identified as hypothetical ones. Comparative analysis indicated that the StenR_269 phage had a similar genome organization to that of the unclassified Xanthomonas phage DES1, despite their low protein similarity. In addition, the signature proteins of StenR_269 and DES1 had low similarity and these proteins clustered far from the corresponding proteins of classified phages. Thus, the StenR_269 genome is orphan and the analyzed data suggest a new family in the class Caudoviricetes.
- Subjects
SOIL remediation; COAL mining; ELECTRON microscopy; SOIL sampling; BACTERIOPHAGES; COMPARATIVE genomics
- Publication
Viruses (1999-4915), 2023, Vol 15, Issue 12, p2437
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v15122437