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- Title
Whole Genome Analysis of Human Rotaviruses Reveals Single Gene Reassortant Rotavirus Strains in Zambia.
- Authors
Maringa, Wairimu M.; Simwaka, Julia; Mwangi, Peter N.; Mpabalwani, Evans M.; Mwenda, Jason M.; Mphahlele, M. Jeffrey; Seheri, Mapaseka L.; Nyaga, Martin M.
- Abstract
Rotarix® vaccine was implemented nationwide in Zambia in 2013. In this study, four unusual strains collected in the post-vaccine period were subjected to whole genome sequencing and analysis. The four strains possessed atypical genotype constellations, with at least one reassortant genome segment within the constellation. One of the strains (UFS-NGS-MRC-DPRU4749) was genetically and phylogenetically distinct in the VP4 and VP1 gene segments. Pairwise analyses demonstrated several amino acid disparities in the VP4 antigenic sites of this strain compared to that of Rotarix®. Although the impact of these amino acid disparities remains to be determined, this study adds to our understanding of the whole genomes of reassortant strains circulating in Zambia following Rotarix® vaccine introduction.
- Subjects
ZAMBIA; HUMAN genome; WHOLE genome sequencing; ROTAVIRUSES; GENES; AMINO acids; ROTAVIRUS diseases
- Publication
Viruses (1999-4915), 2021, Vol 13, Issue 9, p1872
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v13091872