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- Title
Acetylation of lysine 9 on histone H3 is associated with increased pro-inflammatory cytokine release in a cigarette smoke-induced rat model through HDAC1 depression.
- Authors
Chen, Xi; Guan, Xiao-jun; Peng, Xiao-hua; Cui, Zhi-lei; Luan, Chun-yu; Guo, Xue-jun
- Abstract
Objective and design: Cigarette smoke (CS)-induced inflammation is critical in chronic obstructive pulmonary disease (COPD). However, the role of acetylation at histone 3 lysine 9 (H3K9) in COPD inflammation remains unclear. The present study assessed the effect of acetylation of H3K9 on transcription both in rat lungs and in macrophages. Methods: Sprague-Dawley rats were exposed to CS for either 6 or 12 weeks and rat lungs were collected. Rat macrophages were subjected to 20 % cigarette smoke extract (CSE) for 48 h. Results: CS increased MCP-1 and IL-8 expressions at both mRNA and protein levels in rat lungs after 6 and 12 weeks; increased TNF-α and MMP9 expressions at both levels were noted only after 12 weeks. CSE increased these genes expression in macrophages after 48 h exposure. Increased abundance of acetylated H3K9 protein in rat lungs and in macrophages were associated with decreased expression of histone deacetylase-1(HDAC1). Chromatin immunoprecipitation demonstrated increased level of acetylated H3K9 on promoter regions of these genes both in vivo and in vitro. Knockdown of HDAC1 increased these genes mRNA expression. Conclusions: CS increased H3K9 acetylation and subsequently altered the expression of pro-inflammatory mediators and protease genes through HDAC1 depression in CS-induced rat lungs and in macrophages.
- Subjects
ACETYLATION; LYSINE; HISTONES; PHYSIOLOGICAL effects of tobacco; LABORATORY rats; OBSTRUCTIVE lung disease diagnosis
- Publication
Inflammation Research, 2015, Vol 64, Issue 7, p513
- ISSN
1023-3830
- Publication type
Article
- DOI
10.1007/s00011-015-0832-y