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- Title
Imaging the Met Receptor Tyrosine Kinase (Met) and Assessing Tumor Responses to a Met Tyrosine Kinase Inhibitor in Human Xenograft Mouse Models with a [<sup>99m</sup>Tc] (AH-113018) or Cy 5<sup>**</sup> (AH-112543) Labeled Peptide.
- Authors
Jagoda, Elaine M.; Bhattacharyya, Sibaprasad; Kalen, Joseph; Riffle, Lisa; Leeder, Avrum; Histed, Stephanie; Williams, Mark; Wong, Karen J.; Biying Xu; Szajek, Lawrence P.; Elbuluk, Osama; Cecchi, Fabiola; Raffensperger, Kristen; Golla, Meghana; Bottaro, Donald P.; Choyke, Peter
- Abstract
Developing an imaging agent targeting the hepatocyte growth factor receptor protein (Met) status of cancerous lesions would aid in the diagnosis and monitoring of Met-targeted tyrosine kinase inhibitors (TKIs). A peptide targeting Met labeled with [99mTc] had high affinity in vitro (Kd = 3.3 nM) and detected relative changes in Met in human cancer cell lines. In vivo [99mTc]-Met peptide (AH-113018) was retained in Met-expressing tumors, and high-expressing Met tumors (MKN-45) were easily visualized and quantitated using singlephoton emission computed tomography or optical imaging. In further studies, MKN-45 mouse xenografts treated with PHA 665752 (Met TKI) or vehicle were monitored weekly for tumor responses by [99mTc]-Met peptide imaging and measurement of tumor volumes. Tumor uptake of [99mTc]-Met peptide was significantly decreased as early as 1 week after PHA 665752 treatment, corresponding to decreases in tumor volumes. These results were comparable to Cy5** -Met peptide (AH-112543) fluorescence imaging using the same treatment model. [99mTc] or Cy5** -Met peptide tumor uptake was further validated by histologic (necrosis, apoptosis) and immunoassay (total Met, p Met, and plasma shed Met) assessments in imaged and nonimaged cohorts. These data suggest that [99mTc] or Cy5** -Met peptide imaging may have clinical diagnostic, prognostic, and therapeutic monitoring applications.
- Publication
Molecular Imaging, 2015, Vol 14, p499
- ISSN
1535-3508
- Publication type
Article
- DOI
10.2310/7290.2015.00023