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- Title
Calix[4]arene C-90 selectively inhibits Ca,Mg-ATPase of myometrium cell plasma membrane.
- Authors
Veklich, T.; Shkrabak, A.; Slinchenko, N.; Mazur, I.; Rodik, R.; Boyko, V.; Kalchenko, V.; Kosterin, S.
- Abstract
The supramolecular compound calix[4]arene C-90 (5,11,17,23-tetra(trifluoro)methyl(phenylsulfonylimino)-methylamino-25,26,27,28-tetrapropoxycalix[4]arene) is shown to efficiently inhibit the ATP hydrolase activity of Ca,Mg-ATPase in the myometrium cell plasma membrane fraction and also in a preparation of the purified enzyme solubilized from this subcellular fraction. The inhibition coefficient I values were 20.2 ± 0.5 and 58.5 ± 6.4 μM for the membrane fraction and the solubilized enzyme, respectively. The inhibitory effect of calix[4]arene C-90 was selective comparatively to other ATPases localized in the plasma membrane: calix[4]arene C-90 did not influence the activities of Na,K-ATPase and 'basal' Mg-ATPase. The inhibitory effect of calix[4]arene C-90 on the Ca,Mg-ATPase activity was associated with the cooperative action of four trifluoromethylphenyl sulfonylimine (sulfonylamidine) groups oriented similarly on the upper rim of the calix[4]arene macrocycle (the calix[4]arene 'bowl'). The experimental findings seem to be of importance for studies, using calix[4]arene C-90, of membrane mechanisms of regulation of calcium homeostasis in smooth muscle cells and also for investigation of the participation of the plasma membrane Ca-pump in control of electro- and pharmacomechanical coupling in myocytes.
- Subjects
CALIXARENES; SODIUM/POTASSIUM ATPase; ENZYME inhibitors; MYOMETRIUM; CELL membranes; HYDROLASES; HOMEOSTASIS
- Publication
Biochemistry (00062979), 2014, Vol 79, Issue 5, p417
- ISSN
0006-2979
- Publication type
Article
- DOI
10.1134/S0006297914050058