We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Characterization of IAA Production by the Mangrove Cyanobacterium <i>Phormidium</i> sp. MI405019 and Its Influence on Tobacco Seed Germination and Organogenesis.
- Authors
Boopathi, Thangavelu; Balamurugan, Vadivel; Gopinath, Selvaraj; Sundararaman, Muthuraman
- Abstract
The production of indole-3-acetic acid (IAA) by a mangrove root-associated cyanobacterium, Phormidium sp. MI405019, was demonstrated in this study. The extracellular extract (ECE) of this cyanobacterial culture filtrate was tested on tobacco seed germination and callus differentiation. In ECE treatment, seed germination was increased by 40 % when compared to that of control. In addition, ECE also induced multiple roots from tobacco callus. Further, the factors such as concentration of l-tryptophan and NaCl (salinity) on IAA production were studied. IAA in the medium was initially increased up to a certain period and subsequently decreased in all salinity ranges tested. The amount of IAA production was decreased after 48 h in the culture grown in media amended with 2 % NaCl and without NaCl. However, the IAA concentration was increased up to 96 h in media containing 4 % NaCl. IAA produced by Phormidium sp. MI405019 was extracted from the culture filtrate and its identity was confirmed by thin-layer chromatography and ultra-fast liquid chromatography. To the best of our knowledge, this is the first report of IAA production by cyanobacteria isolated from a mangrove ecosystem. Based on the results in this study, the utilization of Phormidium sp. MI405019 in the aspects of mangrove growth promotion was discussed.
- Subjects
INDOLEACETIC acid; MANGROVE plants; CYANOBACTERIA; PHORMIDIUM; GERMINATION; MORPHOGENESIS; TOBACCO
- Publication
Journal of Plant Growth Regulation, 2013, Vol 32, Issue 4, p758
- ISSN
0721-7595
- Publication type
Article
- DOI
10.1007/s00344-013-9342-8