We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Unveiling the Genetic Tapestry: Exploring Rhizoctonia solani AG-3 Anastomosis Groups in Potato Crops across Borders.
- Authors
Naqvi, Syed Atif Hasan; Abbas, Aqleem; Farhan, Muhammad; Kiran, Rafia; Hassan, Zeshan; Mehmood, Yasir; Ali, Amjad; Ahmed, Niaz; Hassan, Muhammad Zeeshan; Alrefaei, Abdulwahed Fahad; Ölmez, Fatih; Yang, Seung-Hwan; Baloch, Faheem Shehzad
- Abstract
The current study was carried out to screen 10 isolates (ARS-01–ARS-10) of Rhizoctonia. solani from potato tubers cv. Kuroda, which were collected from various potato fields in Multan, Pakistan. The isolates were found to be morphologically identical, as the hyphae exhibit the production of branches at right angles and acute angles often accompanied by septum near the emerging branches. Anastomosis grouping showed that these isolates belonged to AG-3. A pathogenicity test was performed against the susceptible Kuroda variety and among the isolates, ARS-05 exhibited the highest mean severity score of approximately 5.43, followed by ARS-09, which showed a mean severity score of about 3.67, indicating a moderate level of severity. On the lower end of the severity scale, isolates ARS-06 and ARS-07 displayed mean severity scores of approximately 0.53 and 0.57, respectively, suggesting minimal symptom severity. These mean severity scores offer insights into the varying degrees of symptom expression among the different isolates of R. solani under examination. PCoA indicates that the severe isolate causing black scurf on the Kuroda variety was AG-3. A comprehensive analysis of the distribution, genetic variability, and phylogenetic relationships of R. solani anastomosis groups (AGs) related to potato crops across diverse geographic regions was also performed to examine AG prevalence in various countries. AG-3 was identified as the most widespread group, prevalent in Sweden, China, and the USA. AG-5 showed prominence in Sweden and the USA, while AG-2-1 exhibited prevalence in China and Japan. The phylogenetic analysis unveiled two different clades: Clade I comprising AG-3 and Clade II encompassing AG-2, AG-4, and AG-5, further subdivided into three subclades. Although AGs clustered together regardless of origin, their genetic diversity revealed complex evolutionary patterns. The findings pave the way for region-specific disease management strategies to combat R. solani's impact on potato crops.
- Subjects
SWEDEN; RHIZOCTONIA solani; SURGICAL anastomosis; CROPS; TAPESTRY; GENETIC variation; POTATOES
- Publication
Plants (2223-7747), 2024, Vol 13, Issue 5, p715
- ISSN
2223-7747
- Publication type
Article
- DOI
10.3390/plants13050715