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- Title
Development of schemes of induced mutagenesis for improving the productivity of Aspergillus strains producing amylolytic enzymes.
- Authors
Kostyleva, E.; Sereda, A.; Velikoretskaya, I.; Burtseva, E.; Veselkina, T.; Nefedova, L.; Sharikov, A.; Tsurikova, N.; Lobanov, N.; Sinitsyn, A.
- Abstract
In order to improve the biosynthesis of amylolytic enzymes by industrial Aspergillus strains, the efficiency of stepwise application of the following methods of induced mutagenesis was studied: ultraviolet (UV) irradiation, gamma irradiation, and treatment with N-methyl- N-nitro- N-nitrosoguanidine (NG). It was found that at the early stages of mutagenesis of the glucoamylase-producing A. awamori strain UV and NG, used either alone or in combination, were efficient enough as mutagenic agents, providing an increase of glucoamylase activity by 30-35 and 50-60%, respectively. At the later stages, serial UV mutagenesis and gamma-irradiation showed high efficiency for both A. awamori, and A. oryzae strains. Gamma-mutagenesis of Aspergillus strains using a cobalt source provided the most stable and highly active strains retaining 90-95% of their activity after five transfers on agar medium. The experiments resulted in significant improvement of the studied industrial strains, more than doubling activity of the target enzymes.
- Subjects
MUTAGENESIS; ASPERGILLUS; ULTRAVIOLET radiation; GLUCOAMYLASE; GAMMA rays; NITROSOGUANIDINE
- Publication
Microbiology (00262617), 2017, Vol 86, Issue 4, p493
- ISSN
0026-2617
- Publication type
Article
- DOI
10.1134/S0026261717040087