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- Title
NITRATE REDUCTION BY WHOLE CELLS OF ANKISTRODESMUS BRAUNII AND CHLAMYDOMONAS REINHARDI.
- Authors
Hipkin, C. R.; Syrett, P. J.
- Abstract
Methods are described for the assay of nitrate reductase in frozen/thawed cells of Ankistrodesmus braunii and Chlamydomonas reinhardi Because Ankistrodesmus nitrate reductase can use NADPH as an electron-donor and because the cells of Ankistrodesmus contain a high concentration of glucose-6-phosphate, it is suggested that, in frozen-thawed cells, nitrate reduction is coupled, through NADPH, to the oxidation of glucose-6-phosphate. With Chlamydomonas cells either exogenous NADH or reduced FMN can act as reductant. The permeability of Chlamydomonas cells can also be increased by glycerol treatment and this makes assay of nitrate reductase possible without freezing and thawing.
- Subjects
ANKISTRODESMUS braunii; CHLAMYDOMONAS; NITRATES; CELLS; OXIDATION; GLUCOSE
- Publication
New Phytologist, 1977, Vol 79, Issue 3, p639
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/j.1469-8137.1977.tb02248.x