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- Title
Oral vaccination of mice with lipid-encapsulated Mycobacterium bovis BCG: Anatomical sites of bacterial replication and immune activity.
- Authors
Aldwell, Frank E.; Baird, Margaret A.; Fitzpatrick, Clare E.; McLellan, Alex D.; Cross, Martin L.; Lambeth, Matthew R.; Buchan, Glenn S.
- Abstract
Lipid microencapsulation of Mycobacterium bovis bacille Calmette–Guérin (BCG) produces an oral delivery vaccine that can establish systemic cell-mediated immune reactivity and protection against aerosol mycobacterial challenge in mice. Here, we describe the lymphatic and mucosal sites of bacterial replication, and location of Mycobacterium-specific IFN-γ-secreting cell populations, following oral vaccination of BALB/c mice. Eight weeks following a single oral dose of lipid-encapsulated BCG, viable BCG organisms were recovered from the mesenteric lymph nodes (MLN) of 11/12 mice investigated (93%). Live bacteria were also occasionally recovered from the cervical lymph nodes (17%) and Peyer's patches (8%), but not from homogenates of the lungs or spleen. Strong Mycobacterium-specific IFN-γ production was recorded among isolated splenocytes, but not among populations of mononuclear cells derived from the MLN or lungs. Oral vaccination of mice with lipid-encapsulated BCG thus appears to promote a state of systemic immunological reactivity more akin to that observed following parenteral rather than conventional oral vaccination, despite the fact that replicating bacilli are restricted to lymphatic tissues of the alimentary tract. Possible patterns of lymphocyte sensitization and trafficking are discussed.
- Subjects
MYCOBACTERIA; BCG vaccines; ORAL vaccines; VACCINATION; IMMUNITY; MICROENCAPSULATION
- Publication
Immunology & Cell Biology, 2005, Vol 83, Issue 5, p549
- ISSN
0818-9641
- Publication type
Article
- DOI
10.1111/j.1440-1711.2005.01369.x