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- Title
Odontoclastogenesis of mouse papilla‐derived MDPC‐23 cells induced by lipopolysaccharide.
- Authors
Li, C.; Qi, W. T.; Jiang, H. W.
- Abstract
Abstract: Aim: To investigate the role of Lipopolysaccharide (LPS) in the odontoclast differentiation of MDPC‐23 cells. It was hypothesized that MDPC‐23 odontoblast‐like cells may function as odontoclasts under the influence of LPS. Methodology: MDPC‐23 cells were cultured in the presence of 0.1 or 1 μg mL−1 LPS for 6 days. Cell viability was determined using the CCK8 assay. TRAP staining, dentine resorption assay and ROS detection by confocal laser scanning microscope were used to test the odontoclast‐like function of the induced cells. In additional, the related protein expression was confirmed by Western blotting and ELISA. An unpaired Student's <italic>t</italic>‐test and one‐way anova were used in statistical analysis<bold>.</bold> Results: TRAP‐positive cells, which are multinucleated, on the dentine slice were significantly increased in 1 μg mL−1 LPS‐induced cells (<italic>P</italic> < 0.05). Osteoclast‐specific proteins such as TRAP cathepsin K and Rac1 were upregulated in the 1 μg mL−1 LPS‐treated cells (<italic>P</italic> < 0.05), whilst the expression of marker proteins of the RANKL‐RANK signalling pathway (RANKL, RANK and TRAF6) in the induced cells was not significantly changed (<italic>P</italic> > 0.05). ROS production was observed in the 1 μg mL−1 LPS treatment group (<italic>P</italic> < 0.05), but no significant differences were observed in the level of RANKL in the cell supernatant between the LPS‐treated group and the control group (<italic>P</italic> > 0.05). Conclusions: A known value of 1 μg mL−1 LPS might induce odontoblast‐like MDPC‐23 cells to generate odontoclast‐like cells or to function as odontoclasts. The data might provide a new explanation for the precursors of odontoclasts and root resorption.
- Subjects
ODONTOCLASTS; PHYSIOLOGICAL effects of lipopolysaccharides; DENTAL pulp; ANALYSIS of variance; OSTEOCLASTS; CELL survival; ROOT resorption (Teeth)
- Publication
International Endodontic Journal, 2018, Vol 51, pe115
- ISSN
0143-2885
- Publication type
Article
- DOI
10.1111/iej.12771