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- Title
Nitric oxide upregulates expression of DNA-PKcs to protect cells from DNA-damaging anti-tumour agents.
- Authors
Xu, Weiming; Liu, Lizhi; Smith, Graeme C. M.; Charles, lan G.
- Abstract
Nitric-oxide synthase (NOS) activity has been detected in many human tumours, although its function is unclear. Here we show that exposure of cells to nitric oxide (NO) results in a 4-5-fold increase in expression of the DNA-dependent protein-kinase catalytic subunit (DNA-PKcs), one of the key enzymes involved in repairing double-stranded DNA breaks. This NO-mediated increase in enzymatically active DNA-PK not only protects cells from the toxic effects of NO, but also provides crossprotection against clinically important DNA-damaging agents, such as X-ray radiation, adriamycin, bleomycin and cisplatin. The NO-mediated increase in DNA-PKcs described here demonstrates the presence of a new and highly effective NO-mediated mechanism for DNA repair.
- Subjects
NITRIC-oxide synthases; TUMORS; NITRIC oxide; PROTEIN kinases
- Publication
Nature Cell Biology, 2000, Vol 2, Issue 6, p339
- ISSN
1465-7392
- Publication type
Article
- DOI
10.1038/35014028