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- Title
In vivo nuclear delivery of oligonucleotides via hybridizing bifunctional peptides.
- Authors
Brandén, L J; Christensson, B; Smith, C I Edvard
- Abstract
Linking proteins directly to nucleic acids has been a complex task. By hybridizing a bifunctional peptide nucleic acid (PNA) consisting of a nucleic acid binding moiety and a nuclear localization signal (NLS) we have previously demonstrated that it is possible to link protein functions directly to nucleic acids containing a PNA target site. By hybridizing fluorescently labeled oligonucleotides to PNA-NLS molecules and subsequently transfecting different organs in vivo we demonstrate an active nuclear translocation of the PNANLS/oligonucleotide complex in different mouse organs.
- Subjects
OLIGONUCLEOTIDES; CHEMOKINES; CHROMOSOMAL translocation
- Publication
Gene Therapy, 2001, Vol 8, Issue 1, p84
- ISSN
0969-7128
- Publication type
Article