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- Title
Selective cell ablation in transgenic mice expressing E. coli nitroreductase.
- Authors
Clark, A J; Iwobi, M; Cui, W; Crompton, M; Harold, G; Hobbs, S; Kamalati, T; Knox, R; Neil, C; Yull, F; Gusterson, B
- Abstract
The gene encoding E. coli nitroreductase (NTR) was expressed in the luminal cells of the mammary gland of transgenic mice using the ovine β-lactoglobulin promoter. Treatment of NTR expressing animals with the prodrug CB1954 (5-aziridin-1-yl-2-4-dinitrobenzamide) resulted in a rapid and selective killing of this population of cells whereas the closely associated myoepithelial cells were unaffected. NTR-mediated inducible cell ablation offers a number of advantages over the use of HSV1-tk for the selective killing of cells in vivo.
- Subjects
TRANSGENIC animals; ESCHERICHIA coli; GENE therapy
- Publication
Gene Therapy, 1997, Vol 4, Issue 2, p101
- ISSN
0969-7128
- Publication type
Article
- DOI
10.1038/sj.gt.3300367