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- Title
Glucose-6-phosphate Dehydrogenase Deficiency: Biochemical and Histochemical Studies on Hair Roots for Carrier Detection.
- Authors
Vermorken, A. J. M.; Wirtz, P.; Spierenburg, G. Th.; van Bennekom, C. A.; de Bruyn, C. H. M. M.; Oei, T. L.
- Abstract
Kinetic properties of human hair root glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase were studied in order to optimize the assay of these enzymes in lysates from single hair roots. In contrast to previously reported methods, an excess of purified 6-phosphogluconate dehydrogenase was added to the glucose-6-phosphate dehydrogenase reaction mixtures, thus allowing a more exact quantification of glucose-6-phosphate dehydrogenase activity. Although enzyme histochemical techniques suggest a similar distribution of hair root glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, enzyme assays on hair root segments after microdissection nevertheless indicate differences in the distribution of these enzymes. Upon storage a gradual drop in the activity of both hair root enzymes was found, but the rate of decrease in enzyme activity was about equal: the enzyme activity ratio was, therefore, not affected. This opens interesting possibilities for mailing hair roots for screening purposes without any special precautions.
- Publication
Clinical Chemistry & Laboratory Medicine, 1979, Vol 17, Issue 5, p325
- ISSN
1434-6621
- Publication type
Article