We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Highly Sensitive Love Mode Acoustic Wave Platform with SiO 2 Wave-Guiding Layer and Gold Nanoparticles for Detection of Carcinoembryonic Antigens.
- Authors
Li, Chong; Zhang, Jikai; Xie, Haiyu; Luo, Jingting; Fu, Chen; Tao, Ran; Li, Honglang; Fu, Yongqing
- Abstract
A highly sensitive and precise Love wave mode surface acoustic wave (SAW) immunosensor based on an ST-cut 90°X quartz substrate and an SiO2 wave-guiding layer was developed to detect cancer-related biomarkers of carcinoembryonic antigens (CEAs). A delay line structure of the SAW device with a resonant frequency of 196 MHz was designed/fabricated, and its surface was functionalized through CEA antibody immobilization. The CEA antibodies were bound with gold nanoparticles and CEA antibodies to form a sandwich structure, which significantly amplified the mass loading effect and enhanced the maximum responses by 30 times. The center frequency of the Love wave immunosensor showed a linear response as a function of the CEA concentration in the range of 0.2–5 ng/mL. It showed a limit of detection of 0.2 ng/mL, and its coefficient of determination was 0.983. The sensor also showed minimal interference from nonspecific adsorptions, thus demonstrating its promise for point-of-care applications for cancer biomarkers.
- Subjects
SOUND waves; ACOUSTIC surface waves; CARCINOEMBRYONIC antigen; GOLD nanoparticles; SANDWICH construction (Materials); TUMOR markers; DELAY lines
- Publication
Biosensors (2079-6374), 2022, Vol 12, Issue 7, pN.PAG
- ISSN
2079-6374
- Publication type
Article
- DOI
10.3390/bios12070536