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- Title
Effects of Heat Stress and Lipopolysaccharides on Gene Expression in Chicken Immune Cells.
- Authors
Yang, Guang; Zhou, Xinyi; Chen, Shutao; Liu, Anfang; Liu, Lingbin; Wang, Haiwei; Wang, Qigui; Lan, Xi
- Abstract
Simple Summary: Poultry are influenced by environmental stimuli, which can impact their productivity and immune function. Exploring the mechanisms behind these effects is crucial for better animal husbandry. By examining previous research, we identified stress-related genes and used microarray technology to measure gene expression levels and pathway enrichment. Ultimately, we confirmed that certain immune genes are inhibited by environmental stress. These findings provide preliminary explanations for the decreased immune function in poultry under stress and offer a theoretical basis for improving actual production. Prolonged exposure to high temperatures and humidity can trigger heat stress in animals, leading to subsequent immune suppression. Lipopolysaccharides (LPSs) act as upstream regulators closely linked to heat stress, contributing to their immunosuppressive effects. After an initial examination of transcriptome sequencing data from individual samples, 48 genes displaying interactions were found to potentially be associated with heat stress. Subsequently, to delve deeper into this association, we gathered chicken bone marrow dendritic cells (BMDCs). We combined heat stress with lipopolysaccharides and utilized a 48 × 48 Fluidigm IFC quantitative microarray to analyze the patterns of gene changes under various treatment conditions. The results of the study revealed that the combination of heat stress and LPSs in a coinfection led to reduced expressions of CRHR1, MEOX1, and MOV10L1. These differentially expressed genes triggered a pro-inflammatory response within cells via the MAPK and IL-17 signaling pathways. This response, in turn, affected the intensity and duration of inflammation when experiencing synergistic stimulation. Therefore, LPSs exacerbate the immunosuppressive effects of heat stress and prolong cellular adaptation to stress. The combination of heat stress and LPS stimulation induced a cellular inflammatory response through pathways involving cAMP, IL-17, MAPK, and others, consequently leading to decreased expression levels of CRHR1, MEOX1, and MOV10L1.
- Subjects
GENE expression; CHICKENS; LIPOPOLYSACCHARIDES; BONE marrow cells; ESTRUS
- Publication
Animals (2076-2615), 2024, Vol 14, Issue 4, p532
- ISSN
2076-2615
- Publication type
Article
- DOI
10.3390/ani14040532